Effects of spraying exogenous hormone 6-benzyladenine (6-BA) after waterlogging on grain yield and growth of summer maize

Abstract

Cytokinin is known to be involved in the regulation of plant responses to counteract the adverse effects of stress conditions. This work investigated the effects of exogenous spraying of 6-benzyladenine (6-BA) after water logging for 6 days at the third leaf stage (V-3WL+BA) and the sixth leaf stage (V-6WL+BA) on grain yield and growth of summer maize hybrids DengHai605 (DH605) and ZhengDan958 (ZD958), which are planted most popularly in China. Waterlogging under field conditions delayed the growth process, decreased leaf area index, chlorophyll content and dry matter accumulation, resulted in a significant reduction of grain yield with a decrease of 30.30% and 18.78% under V-3WL (water logging for 6 days at the third leaf stage) and V-6WL (waterlogging for 6 days at the sixth leaf stage) of DH605, 30.39% and 21.38% under V-3WL and V-6WL of ZD958, respectively, compared to the control (CK). By contrast, exogenous application of 6-BA effectively alleviated the adverse effects of waterlogging on summer maize by delaying leaf senescence, increasing chlorophyll content and improving photosynthetic performance. At tasseling stage, the Pn of V-3WL+BA and V-6WL+BA in DH605 increased by 9.57% and 8.38%, respectively, compared to that of V-3WL and V-6WL, while the corresponding values for ZD958 increased by 2.96% and 4.05%, respectively. Those responses are conducive to the accumulation of photosynthetic products, increasing grain filling rate, and improving ear character, and then leading to increase grain yield of summer maize. Grain yield of DH605 under V-3WL+BA and V-6WL+BA increased by 15.57% and 12.39%, respectively, compared to that of V-3WL and V-6WL, while the corresponding values for ZD958 increased by 20.05% and 15.32%, respectively. Clearly, spraying exogenous 6-BA could effectively improve grain-filling characteristics and photosynthesis of waterlogged summer maize, resulting in a significant increase of grain yield, compared to water logging treatments.