EFFECTS OF DIFFERENT CONCENTRATIONS OF TITANIUM OXIDE NANOPARTICLES AND BULK ON GERMINATION CHARACTERISTICS AND ANTIOXIDANT ENZYMES ACTIVITY OF AVENA SATIVA L. SEEDS

Abstract

The aim of this study is to compare the effects of different concentrations of nano and bulk titanium oxide on germination characteristics and the activity of antioxidant enzymes in Avena Sativa L. The titanium oxide nanoparticles diameter was determined about 40 nm using AFM, DLS, XRD analysis. In this study, based on a completely randomized design, seeds were placed in Petri dishes containing different concentrations of nano and bulk titanium oxide (50, 150, 250, 350 ppm). Three replications were considered for each concentration and the germination factors and antioxidant enzyme activity in the treated plant were compared with the control. The average data were compared using one-way ANOVA analysis and DUNCAN tests. According to the results, the following parameters of seedling vigor index, root length, dry weight, wet weight, guaiacol peroxidase enzyme, and polyphenol oxidase were statistically significant. The highest germination percentage, relative germination percentage, germination rate, weight germination index and shoot length were observed at 250 ppm bulk titanium oxide. In addition, the highest average germination time, seedling vigor index, optimum seedling index, and root length were observed in 50 ppm bulk titanium oxide treatment and the highest shoot length was observed in 250 ppm nano-titanium oxide treatment. In the enzyme activity, the highest activity of guaiacol peroxidase enzyme was observed in 150 ppm bulk titanium oxide treatment and the highest Polyphenol oxidase enzyme activity was observed in bulk titanium oxide 350 ppm treatment.