Effects of different concentrations of aluminum hydroxide adjuvant and adsorption methods on the immunogenicity of diphtheria-tetanus-acellular pertussis and inactivated poliovirus combined vaccine

Abstract

Objective To investigate the effects of different concentrations and adsorption methods of aluminum hydroxide adjuvant produced by different manufacturers on the immunogenicity of the diphtheria-tetanus-acellular pertussis and inactivated poliovirus combined vaccine (DTaP-sIPV). Methods Five antigens of DTaP were adsorbed onto different concentrations (0.42 mg/ml, 0.47 mg/ml and 0.52 mg/ml) of aluminum hydroxide from different manufacturers through sequential and separate adsorption. Adsorbability, anti-pertussis toxin (PT)/filamentous hemagglutinin (FHA)/pertactin (PRN)/diphtheria toxoid (DT)/tetanus toxoid (TT) antibodies and the potency of vaccines were detected. Results The adsorbability of aluminum hydroxide adjuvant slightly decreased with the reduction of concentration. No significant difference in potency and antibody level was observed between sequential and separate adsorption. Moreover, no significant difference in antibody level was observed between vaccines prepared with aluminum hydroxide adjuvant produced by General Chemical Corp and our institute. Conclusion Aluminum hydroxide adjuvant produced by our institute at the concentration of 0.52 mg/ml and separate adsorption method are suitable for preparation of DTaP-sIPV. Key words: DTaP-sIPV; Adsorbability; Vaccine potency; Anti-pertussis toxin (anti-PT) antibody; Anti-filamentous hemagglutinin (anti-FHA) antibody; Anti-pertactin (anti-PRN) antibody; Anti-diphtheria toxoid (anti-DT) antibody; Anti-tetanus toxoid (anti-TT) antibody