Abstract
Simple SummaryThis study aimed to determine the effects of dietary vibroactivated clinoptilolite supplementation on the intramammary microbiological findings in dairy cows, causative agents of intramammary infection, and their sensitivity to antibiotics. The cows (n = 78) were randomly divided into two groups: the clinoptilolite (CPL)-treated group that received 100 g of clinoptilolite (CPL) in-feed (n = 38) from the seventh month of pregnancy to 75 days after calving and the control group (CON) of untreated cows (n = 40). Milk samples were taken from each cow on days 7, 25, 45, and 75 postpartum. Different causative pathogens were isolated in 86 udder quarters (7.07%), in 3.87% environmental microflora, and 89.06% were bacteriologically negative. The most effective antibiotics were cefoperazone and amoxicillin-clavulanic acid, while cloxacillin and tetracycline were the least effective. In the CPL group (14 cows), nine pathogens were isolated in 27 quarters, while in the control (CON) group (24 cows), 13 pathogens in 59 quarters. Cows from the CON group had a 1.96 times higher risk of intramammary infection than cows from the CPL group.The aim of this study was to determine the effects of dietary vibroactivated clinoptilolite supplementation on the intramammary microbiological findings in dairy cows, causative agents of potential intramammary infection, and their sensitivity to antibiotics. Cows (n = 78) were randomly divided into two groups: CPL-treated group that received clinoptilolite (CPL) in-feed (n = 38), i.e., 50 g natural powdered zeolite CPL, twice daily from the seventh month of pregnancy to 75 days after calving, and the control group (CON) of untreated cows (n = 40). Milk samples were taken from each cow on days 7, 25, 45, and 75 postpartum. The following causative pathogens were isolated in 86 udder quarters: Staphylococcus aureus in 5.81% of positive samples, Staphylococcus spp. 9.32%, coagulase-negative Staphylococcus (CNS) 22.09%, Streptococcus uberis 13.95%, Streptococcus agalactiae 1.16%, Streptococcus sp. 3.49%, Escherichia coli 8.13%, Enterococcus spp. 6.98%, Corynebacterium spp. 11.63%, Pasteurella sp. 10.47%, Serratia spp. 2.33%, and Arcanobacterium pyogenes, Citrobacter sp., Prototheca sp., and yeasts each in 1.16% of samples. Additionally, 3.87% of environmental microflora samples (n = 47) and 89.06% of udder samples (n = 1083) were bacteriologically negative. The most effective antibiotics were cefoperazone and amoxicillin-clavulanic acid, while cloxacillin and tetracycline were the least effective antibiotics in both groups. In the clinoptilolite supplemented (CPL) group (n = 38) of 14 cows, nine causative agents of mastitis were isolated in 27 quarters, while in the control (CON) group (n = 40) of 24 cows, 13 causative agents of mastitis were isolated in 59 quarters. Cows from the CON group had a 1.96 times higher risk of intramammary infection than cows from the CPL group during the observation period (odds ratio = 1.96, p = 0.0031; 95% CI = 1.2570–3.0770).
Highlights
Zeolites are natural, hydrated, crystalline aluminosilicates composed of SiO4 and AlO4 linked together by oxygen atoms into three-dimensional frameworks, like a honeycomb structure with microspores
The following causative pathogens were isolated in 86 udder quarters: Staphylococcus aureus in 5.81% of positive samples, Staphylococcus spp. 9.32%, coagulase-negative Staphylococcus (CNS) 22.09%, Streptococcus uberis 13.95%, Streptococcus agalactiae
Group (n = 38) of 14 cows, nine causative agents of mastitis were isolated in 27 quarters, while in the control (CON) group (n = 40) of 24 cows, 13 causative agents of mastitis were isolated in 59 quarters
Summary
Zeolites are natural, hydrated, crystalline aluminosilicates composed of SiO4 and AlO4 linked together by oxygen atoms into three-dimensional frameworks, like a honeycomb structure with microspores. A negative framework charge caused by the presence of aluminium is balanced by the exact number of cations required to make it neutral. These cations are not part of the zeolite network, and can be replaced by other cations [1]. Among the many types of natural zeolites, clinoptilolite (CPL), which incorporates a biologically active nanoporous structure, is the most widespread and scientifically studied [3]. It is assumed that CPL might modulate metabolic, endocrine, and antioxidative status in dairy cows and improve their health, fertility, and milk production [7]
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