Abstract
Amino acids can act as signaling molecules in protein synthesis and animal growth by regulating several genes in the target of rapamycin (TOR) pathway. An 8-week feeding trial was conducted to examine the effects of dietary proline on growth performance, body composition, amino acid profiles, blood-biochemistry and expression of TOR pathway-related genes in juvenile Nibea diacanthus. Fish (450 juvenile N. diacanthus) were assigned to six dietary proline supplementation groups (0, 2.5, 5, 7.5, 10 and 12.5 g kg−1 of dry diet). The results revealed that the growth performance and feed utilization of N. diacanthus were not significantly improved with dietary proline level (P > 0.05). Whole-body crude protein and some amino acids in muscle tissue were significantly (P < 0.05) affected by the supplemental dietary proline, while the levels of hepatic GOT and GPT increased significantly (P < 0.05) with increasing levels of dietary proline. The transcript level of TOR in muscle was significantly affected by dietary proline level, while the mRNA levels of 4EBP, S6K1, TSC and GTPBP in muscle were relatively unaffected. In conclusion, our results indicate that (1) dietary proline promotes protein synthesis, and (2) proline may promote protein synthesis by regulating the transcription of TOR but not 4EBP, S6K1, TSC and GTPBP.
Published Version
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