Effects of dietary methionine and taurine supplementation to low-fish meal diets on growth performance and oxidative status of European sea bass (Dicentrarchus labrax) juveniles

Abstract

This study aimed to evaluate the effects of dietary methionine (Met) and taurine (Tau) supplementation on growth performance, feed utilization, and oxidative status of European sea bass juveniles. For that purpose, a 12-week growth trial was performed to test four isolipidic (18%) and isonitrogenous (6.7%) practical diets, containing 82% of the protein from plant origin and 18% from fish meal. Diets were formulated to have a Met level below (LMet) or above (HMet) requirements and were supplemented or not with 1% Tau (LMetTau and HMetTau). No dietary Met or Tau effects were observed on growth, feed utilization, and whole-body composition. Increasing dietary Met level led to increased catalase (CAT) and glutathione peroxidase (GPX) activities in the liver and total (tGSH) and reduced (GSH) glutathione in the intestine, while decreasing glutathione reductase (GR) and GPX activities in the intestine. Most of these effects were only observed in fish fed diets not supplemented with Tau. Dietary Tau supplementation decreased intestinal tGSH, GSH and oxidised (GSSH) glutathione content, the activity of GPX both in liver and intestine, and glucose-6-phosphate dehydrogenase (G6PDH) activity in the liver. Dietary Tau supplementation also affected hepatic CAT and intestinal GR activities, but effects were dependent of the dietary Met level. Overall, European sea bass seems to cope well with a plant-protein rich diet without Met or Tau supplementation. Both dietary Met and Tau were shown to modulate fish antioxidant response but without altering the oxidative damage level.