Abstract
ABSTRACTPurposeTo evaluate the effect of creatine supplementation in the diet of rats subjected to ischemia and reperfusion of hind limbs.MethodsEighteen male Wistar rats were randomized to receive dietary creatine supplementation (G1) or no supplementation (G2), before being subjected to 4 h of ischemia followed by 4 h of reperfusion. In addition, 10 rats (G3) underwent the same surgical procedure, without ischemia, but with supplementation. After reperfusion, kidney and musculature were evaluated for histological damage and serum levels of alanine aminotransferase, urea and creatinine were obtained.ResultsThe urea dosage showed significant differences between the groups (averages G1 = 155.1; G2 = 211.27; G3 = 160.42). Histological analysis found significant differences between G1 and G2 (but not between G1 and G3) in renal myoglobin cylinders and vacuolar degeneration variables and in hypereosinophilia and karyopyknosis variables in muscle fibers. There were no significant differences in the other variables studied.ConclusionsCreatine supplementation was related to fewer histological lesions, as well as lower levels of plasma urea, which may suggest a protective effect against lesions caused by ischemia and reperfusion of posterior paws muscles in Wistar rats.
Highlights
Myopathic-nephrotic-metabolic syndrome or reperfusion syndrome contributes significantly to the increase in morbidity and mortality from ischemic injuries of several organs[1,2,3]
The aim of this study was to evaluate the effects of dietary creatine supplementation on kidney and striated skeletal musculature of rats submitted to ischemia and reperfusion of hind limbs
Twenty-nine animals aging 10 months were randomly divided by batch into three groups: experiment group, nine animals, which received creatine monohydrate supplementation (Creatine Monohydrate Micronized – Atlhetica Nutrition, Matão/SP) in the dose 2 g diluted in 500 mL of water 5 days before being subjected to a period of 4 h of ischemia and 4 h of reperfusion; control group, 10 animals that did not receive creatine supplementation with the same period of ischemia– reperfusion; and sham group, 10 animals that received supplementation but were not subjected to ischemia–reperfusion in order to verify if creatine supplementation could cause changes in the studied variables[12,13]
Summary
Myopathic-nephrotic-metabolic syndrome or reperfusion syndrome contributes significantly to the increase in morbidity and mortality from ischemic injuries of several organs[1,2,3]. These injuries result from oxidative stress and the inflammatory response, which appear after intervals of only 30 min, and irreversible changes in skeletal muscle occur after 4 to 6 h4,5. It is essential to investigate strategies that can be used at the time of reperfusion to prevent this type of injury, including creatine[7,8]. Its supplementation with a high dose (300 mg/kg/day of body weight) for a period of five to seven days leads to a rapid increase of intramuscular creatine, improving the working capacity of skeletal muscles and delaying the onset of muscle fatigue[9]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
