Biochemical study of the effects of cilostazol in rats subjected to acute ischemia and reperfusion of hind limbs

Sylvio Sebastião De Souza Júnior,Antonio Augusto Moreira Neto,Olavo Ribeiro Rodrigues,José Benedito Dias Lemos,Aurelino Fernandes Schmidt Júnior

doi:10.1590/s0102-86502013000500007

Sylvio Sebastião De Souza Júnior, Antonio Augusto Moreira Neto + Show 3 more

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https://doi.org/10.1590/s0102-86502013000500007

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Abstract

To investigate whether cilostazol has a protective effect on acute ischemia and reperfusion of hind limbs of rats through study of biochemical variables in blood and urine. Forty six animals were randomized and divided into two groups. Group I received a solution of cilostazol (10 mg/Kg) and group II received saline solution 0.9% (SS) by orogastric tube after ligature of the abdominal aorta. After four hours of ischemia the animals were divided into four subgroups: group IA (Cilostazol): two hours of reperfusion. Group IIA (SS): two hours of reperfusion. Group IB (Cilostazol): six hours of reperfusion. Group IIB (SS) six hours of reperfusion. After the reperfusion period, was held to collect urine and blood for biochemical measurements. The biochemical parameters studied were: urea, creatinine, sodium, potassium and myoglobin in blood and urea, creatinine, myoglobin in urine. There was no statistically significant difference between groups. Cilostazol had no protective effect on ischemic acute reperfusion of hind limbs of rats in this model.

Highlights

Sylvio Sebastião de Souza JúniorI, Antonio Augusto Moreira NetoII, Aurelino Fernandes Schmidt JúniorIII, José Benedito Dias
Cilostazol had no protective effect on ischemic acute reperfusion of hind limbs of rats in this model
With the acute ischemia, initiating anaerobic metabolism, glycogen with transformation into lactate, leading to decreased production of adenosine triphosphate (ATP), which alters the permeability of the cell muscle, allowing the output of potassium and myoglobin and entry Sodium and calcium célula[3]

Summary

Introduction

Sylvio Sebastião de Souza JúniorI, Antonio Augusto Moreira NetoII, Aurelino Fernandes Schmidt JúniorIII, José Benedito Dias. Fellow PhD degree, Postgraduate Program in Biomedical Engineering, UMC, Mogi das Cruzes-SP, Brazil. Conception, design, intellectual and scientific content of the study. PhD, Assistant Professor, Department of Surgery, Vascular Surgery Division, UMC, Mogi das Cruzes-SP, Brazil. PhD, Assistant Professor, Department of Surgery, Thoracic Surgery Division, UMC, Mogi das Cruzes-SP, Brazil. PhD, Assistant Professor, Department of Surgery and Traumatology, Surgical Techniques and Experimental Surgery Division, Sao Paulo University (USP), Sao Paulo-SP, Brazil.

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