Abstract
The present study investigated the effects of dietary anthocyanin on the growth performance, haematological, non-specific immune, and spleen gene expression responses of Nile tilapia, Oreochromis niloticus. Five experimental groups of fish with mean weights of 8.24 ± 0.64 g were used in the study; four of these were fed with diets incorporating anthocyanin (20 mg kg -1, 40 mg kg−1, 80 mg kg−1 and 160 mg kg−1), while the fifth was a control group without dietary anthocyanin. Growth performance and haematological parameters of tilapia were not affected by anthocyanin-supplemented diets (p > 0.05). Dietary anthocyanin significantly increased respiratory burst activity, phagocytic activity, phagocytic index, lysozyme activity, myeloperoxidase activity, serum total superoxide dismutase (T.SOD) activity, and serum catalase (CAT) activity (p < 0.05). The total immunoglobulin level was highest in the 80 mg kg−1 group compared with the other groups (p < 0.05). In addition, with the anthocyanin-containing diets, the gene levels of interleukin 1, beta (IL-1β), interleukin 8 (IL-8), tumor necrosis factor (TNF-α), heat shock protein 70 (HSP70), and interferon gamma (IFN-γ) were increased in the fish spleen, and the gene levels of CAT, GPx, and SOD were also increased in fish liver (p < 0.05). At the end of the experiment, the fish were subjected to ammonia stress. The groups fed with 20 and 40 mg kg−1 anthocyanin exhibited higher survival rates than the other groups. In summary, feeding Nile tilapia with anthocyanin-containing diets caused increases in the innate immune parameters, gene expression responses, and the survival rate of the fish subjected to ammonia stress.
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