Effects of deoxyribonucleoside 5′-monophosphates on human skin conditions

Abstract

Vol. 43, No.3 (2009) pp.209–216 We investigated the effects of deoxyribonucleoside 5′-monophosphates (dNMP) on human skin conditions. dNMPs (dAMP, dGMP, dCMP, dTMP) were prepared from salmon milt DNA using enzymatic and chromatographic techniques. First, we investigated the rubbing test of dNMP mixture on skin. 1% of dNMP mixture-containing cream (1%-dNMP group), 3% of dNMP mixture-containing cream (3%-dNMP group), or no dNMP-containing cream (control group) was rubbed on the skin of the face and hand of the male subjects (ten / each group) for 12 weeks. The 1%-dNMP group had significantly improved skin-elasticity compared to that of the control group at 8 weeks, and the tendency of improvement of skin-elasticity was also recognized at 4 and 12 weeks. The 3%-dNMP group tended to have improved skin-elasticity at every period. The water content of face skin was significantly increased in the 3%-dNMP group at 12 weeks. In addition, in the questionnaire, more than 80% of the 3%-dNMP group answered that they felt the improvement of skin roughness after 4 weeks. These results indicated that the dNMP mixture has the effect of improving skin conditions by rubbing. Next, we evaluated the effect of the enzymatic digested DNA-Na which contained dNMPs using normal dermal fibroblast from human skin (SF-TY cell). Enzymatic digested DNA-Na showed the activation effect of cell proliferation and the promoting effect of collagen production on SF-TY cell. In a comparison of dNMPs, dAMP showed the highest activity of collagen production.