Effects of Dark Treatment on Lignin and Cellulose Synthesis in Celery

Abstract

To clarify the impact of continuous dark stress on lignin and cellulose synthesis in celery, shade-tolerant celery varieties were screened. Yellow celery variety ‘Qianhuang No.1’ and green celery variety ‘Qianlv No.1’ were separately grown in vegetable greenhouses. Dark treatments were applied using PVC shading sleeves for 4, 8, 12, and 16 d after celery had grown 10–13 true leaf blades. This study aimed to investigate the impact of varying periods of dark treatment on the morphological characteristics, lignin accumulation, and cellulose accumulation in celery. The results showed that dark treatment led to celery yellowing, a reduced stem thickness, and an increased plant height. Analysis of lignin and cellulose contents, as well as the expression of related genes, showed that dark treatment caused down-regulation of AgLAC, AgC3′H, AgCCR, AgPOD and AgCAD genes, leading to changes in lignin accumulation. Dark treatment inhibited the expression of the AgCesA6 gene, thus affecting cellulose synthesis. Under dark conditions, the expression of AgF5H and AgHCT genes had little effect on lignin content in celery, and the expression of the AgCslD3 gene had little effect on cellulose content. Analysis of morphological characteristics, lignin accumulation and cellulose accumulation after different lengths of dark treatment demonstrated that ‘Qianlv No.1’ is a shade-tolerant variety in contrast to ‘Qianhuang No.1’.