Effects of cypermethrin on the hepatic transcriptome and proteome of the red claw crayfish Cherax quadricarinatus

Abstract

Cypermethrin (CYP) is a synthetic pyrethroid broadly used for pest control, however, it is extremely toxic to aquatic organisms. To assess the toxicity of CYP in red claw crayfish Cherax quadricarinatus, transcriptional and proteomic approaches combining two-dimensional polyacrylamide gel electrophoresis and tandem mass spectrometry were used to compare the hepatic expression profiles. A total of 41,349 unigenes and 8839 differentially expressed genes (DEGs) were obtained, which were enriched in the process. The category of 779 (0.625 ng L−1 CYP vs Con), 1963 (1.25 vs Con), and 2066 (1.25 vs 0.625) DEGs were screened. All findings suggested that CYP can induce antioxidant and biotransformation modulation variations in C. quadricarinatus to resist immunotoxicity and oxidative damages. The category of 196 (0.625 ng L−1 CYP vs Con) specific proteins were differentially expressed: 24 proteins were upregulated, and 20 proteins were downregulated relative to CYP. Protein identification indicated the KEGG pathways of the human immunodeficiency virus 1 infection, insulin signaling pathway, and influenza A enriched. From the differential expression of the selected nine proteins, the increased Loc113824800, Rps19, Atp2, Rps10, Hsp40, Brafldraft_124327, and the decreased Loc117331934, Loc113213835, and Loc106806551 revealed. While for the verification of the eight genes in transcriptome and the above nine genes in proteomic, specifically, gpx5, ggt, loc106458463, chelonianin decreased in the 0.625 ng L−1 CYP group. The transcripts of loc113816050, akr1d1 and gst, chelonianin and loc108675455 decreased and increased in the 1.25 ng L−1 CYP group, respectively. The present study reflects the overall change in cellular structure and metabolism related to the resistance of pyrethroid insecticides.