Abstract
Abstract The stimulatory effects of the mycobacterial polysaccharides MMP (3-O-methylmannose containing polysaccharide) or MGLP (lipopolysaccharide containing 6-O-methylglucose and glucose) on the activity of the fatty acid synthetase from Mycobacterium phlei are mimicked by α-, β-, and γ-cyclodextrins in the order α g β g γ. Methylation to the hexakis or heptakis (2,6-di-O-methyl) α- and β-cyclodextrins increases the stimulatory effect severalfold. Complex formation between the cyclodextrins or their alkyl derivatives and palmitoyl coenzyme A has been demonstrated by Sephadex chromatography and nuclear magnetic resonance spectroscopy.
Highlights
The mycobacterial polysaccharides MGLP’ (I) and MMP [2, 3] markedly stimulate fatty acid synthesis catalyzed by the multienzyme complexes from yeast [4]) Corynebacterium diphtheriae [4], and Nycobacterium phlei [3, 5]
The underlying assumption is that the mode of action of the cyclodextrins and of the mycobacterial polysaccharides is the same; they bind, to a different degree, free or enzyme-bound palmitoyl-CoA and thereby relieve negative feedback inhibition of fatty acid synthesis [4, 6]
The molar ratios of cyclodextrins (a, p, and y-) and palmitoyl-CoA in the complexes are consistent with the “inclusion” model postulated previously for adducts of this type [9, 10]
Summary
The mycobacterial polysaccharides MGLP’ (I) and MMP [2, 3] markedly stimulate fatty acid synthesis catalyzed by the multienzyme complexes from yeast [4]) Corynebacterium diphtheriae [4], and Nycobacterium phlei [3, 5]. Effects of the three cyclodextrins on fatty acid synthesis and to compare their relative binding capacities for long chain acyl- All three cyclodextrins enhance fatty acid synthesis catalyzed by the M. phlei synthetase and do so in the order cy- > p- > y-cyclodextrin.
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