International Journal of Ophthalmology | VOL. 15
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Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells

Publication Date Jun 18, 2022

Abstract

AIM: To investigate the effects of curcumin (Cur) nanoparticles loaded with chitosan derivatives grafted by deoxycholic acid (Chit-DC) on human retinal pigment epithelial (hRPE) cell proliferation and vascular endothelial growth factor (VEGF) mRNA expression. METHODS: Cur nanoparticles were synthesized with Chit-DC as the carrier and Cur as the supported drug. Cell counting kit-8 (CCK-8) method was used to detect the effects of different concentrations of Cur/Chit-DC, Chit-DC, and Cur on the proliferation of hRPE cells for different times. The changes of Cur/Chit-DC and Cur on hRPE cell cycle were determined by flow cytometry. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the mRNA expression levels of VEGF in hRPE cells treated with Cur, Chit-DC and Cur/Chit-DC at 10 μg/mL for 24h. RESULTS: The chitosan derivative nanoparticles, prepared by mixing Cur and Chit-DC, were yellow. The drug released from the nanoparticles reached equilibrium after 96h, and the cumulative drug release amount was 31.6%. Different concentrations of Chit-DC nanoparticle treated hRPE cells had no significant difference in terms of optical density (OD) values compared with the control group at 24h and 48h. Moreover, there was no change in the cell morphology under a light microscope. After 24h treatment with Cur/Chit-DC and Cur, the percentage of G0-G1 phase cells increased and the percentage of S phase cells decreased in all concentration groups. Cur/Chit-DC and Cur in all concentration groups inhibited ...

Concepts
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hRPE Cell
Effects Of Curcumin Nanoparticles
Human Retinal Pigment Epithelial Cell
hRPE Cells
Cur Nanoparticles
Expression Level Of Vascular Endothelial Growth Factor mRNA
Vascular Endothelial Growth Factor
Proliferation Of hRPE Cells
Expression Levels Of Vascular Endothelial Growth Factor
Terms Of Optical Density

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