Effects of Collagen Grafting on Cell Behaviors in BCP Scaffold with Interconnected Pore Structure

Dong-Jun Yang,Sukyoung Kim,Sun-Young Lee,Hyun-Wook An,Kwang-Bum Park,Jae-Hui Jeon,Keun Oh Park

doi:10.1186/s40824-016-0049-3

Abstract

BackgroundThis study was to investigate the effect of collagen grafted porous biphasic calcium phosphate (BCP) on cell attachment, proliferation, and differentiation. Porous BCP scaffolds with interconnected micropore structure were prepared with were prepared and then grafted with a collagen type I. The hydroxyapatite (HA) and β-tricalcium phosphate (TCP) ratio of the TCP scaffolds was about 60/40 and the collagen was crosslinked on the TCP scaffold surface (collagen-TCP).ResultsThe sintered BCP scaffolds showed fully interconnected micropore structures with submicron-sized grains. The collagen crosslinking in the scaffolds was conducted using the the N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (NHS) crosslinking method. The cell proliferation of collagen-BCP scaffolds showed a similar result to that of the BCP scaffolds. However, osteoblastic differentiation and cell attachment increased in the collagen-BCP scaffolds.ConclusionsCollagen-BCP scaffold improved the cell attachment ability in early phase and osteoblastic differentiation.

Highlights

This study was to investigate the effect of collagen grafted porous biphasic calcium phosphate (BCP) on cell attachment, proliferation, and differentiation
This study aimed to investigate the cell behaviors such as cell attachment, proliferation, and differentiation in porous BCP ceramics
Characterization of BCP and collagen-BCP Scaffolds The crystallinity and phase composition in pure BCP and collagen-BCP scaffolds were investigated by using X-ray diffraction (XRD)

Summary

Introduction

This study was to investigate the effect of collagen grafted porous biphasic calcium phosphate (BCP) on cell attachment, proliferation, and differentiation. Xenograft, and synthetic grafting bone substitutes with diverse chemical compositions are widely used as an alternative to autogenous grafting material to repair osseous defects in dentistry [1,2,3,4,5]. The calcium phosphate such as hydroxyapatite (HA), β-tricalcium phosphate (β-TCP) and biphasic calcium phosphate (BCP) are commonly used as a bone substitute due to their excellent biocompatibility. The most synthetic BCP bone substitute consists of a mixture of HA and β-TCP with various ratio. BCP with various HA/TCP ratio shows greater new bone formation when compared with HA and β-TCP

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