Abstract
The in vitro effect of cold shock on erythrocytes of the benthic scorpionfish Scorpaena porcus L. was studied. The blood samples were exposed to 22°С (control group) and 4°С (experimental group) for 3 hours. Cold shock had no significant impact on erythrocyte scorpionfish vital function. The cells maintained the integrity of cytoplasmic membranes, mitochondrial potential and the level of oxidative processes, that the constant values of Propidium Iodide (PI), Rhodamine 123 (R123) and 2',7'-Dichlorodihydrofluorescein diacetate (DCF-DA) fluorescence levels demonstrated. Main changes referred to erythrocyte shape and nucleus functional activity. Cells gained more roundish shape, nucleus size increased with the growth of the nuclear-cytoplasmic ratio (NCR) and the decrease of fluorescence intensity of the cells labeled with SYBR Green I. It appears to be related to the activation of transcriptomic processes.
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