Effects of Cluster of Differentiation 147 Expression Down-Regulation on Proliferation Migration and Invasion of Cervical Cancer Cells

Abstract

To assess the effects of cluster of differentiation 147 on the proliferation, migration and invasion of cervical cancer HeLa cells by inhibiting its expression using short hairpin ribonucleic acid. A total of 110 cases of cervical tissues, including 20 cases of normal cervical epithelial tissues and 90 cases of cervical cancer tissues (30 cases of cervical cancer in situ tissues and 60 cases of cervical squamous cell cancer tissues) stored in our hospital from February 2016 to February 2019 were selected. The expression of cluster of differentiation 147 was detected by immunohistochemistry. Then the correlations of cluster of differentiation 147 expression with clinicopathological parameters such as age, tumor size, invasion depth, clinical stage and lymphatic metastasis of cervical cancer patients were analyzed. Control and experimental groups were transfected with green fluorescent protein-short hairpin ribonucleic acid-cluster of differentiation 147-negative control and green fluorescent protein-short hairpin ribonucleic acid-cluster of differentiation 147, respectively and blank cells were set as normal group. Cell proliferative ability was measured through 5-ethynyl-2'- deoxyuridine labeling assay and the migratory and invasive abilities of the cells were determined using Transwell assay. Western blotting was performed to detect the protein expressions of monocarboxylate transporter 1 and cluster of differentiation 147. The positive expression rate of cluster of differentiation 147 in cervical cancer in situ tissues significantly increased compared with that in normal cervical tissues, which was significantly higher in cervical squamous cell cancer tissues (p<0.05). The expression of cluster of differentiation 147 was not associated with the age and clinical stage of cervical cancer patients (p>0.05), but had close correlations with the tumor size, invasion depth or lymphatic metastasis of cervical cancer patients (p<0.05). Compared with normal group, the proliferative, migratory and invasive abilities of cells were significantly weakened and the protein expressions of monocarboxylate transporter 1 and cluster of differentiation 147 were significantly down-regulated in experimental group (p<0.05). The expression of cluster of differentiation 147 is increased in cervical cancer tissues and inhibiting such expression can prominently decrease the proliferative, invasive and migratory abilities of tumor cells in vitro and in vivo.