Abstract
BackgroundCervical cancer is a major cause of death in women worldwide. Interferon-induced transmembrane protein 1 (IFITM1) is involved in antivirus defense, cell adhesion, and carcinogenesis in different tissues. However, the role of IFITM1 gene in cervical squamous cell cancer is unclear.MethodsTo explore the role of IFITM1 in carcinogenesis of cervical cancer, we investigated the expression of IFITM1 gene in cervical squamous cell carcinoma. IFITM1 mRNA level was measured by real-time quantitative RT-PCR in cervical cancer tissues and their adjacent normal tissues. IFITM1 protein level was measured by immunohistochemistry. Methylation in the IFITM1 gene promoter was detected by methylation-specific PCR. We then transfected HeLa cells with IFITM1 expression vector or control vector. IFITM1 expression was examined; cell migration and invasion were analyzed by wound healing assay and matrigel-coated transwell migration assays, respectively. HeLa cell proliferation was measured by cell counting kit-8 assay and cell cycle analysis. Cell apoptosis was analyzed by Annexin V/propidium iodide double staining assay.ResultsThe difference in IFITM1 protein expression between samples from chronic cervicitis and cervical carcinoma was statistically significant (P < 0.01). Ki-67 and PCNA protein expression levels were significantly higher in cervical cancer tissues than in their corresponding cervicitis tissues (P < 0.05 and P < 0.001, respectively). IFITM1 mRNA level was significantly lower in cervical cancer tissues than in normal cervical tissues (P < 0.05). Methylation of the IFITM1 gene promoter was significantly higher in cervical cancer than in normal cervical tissues (P < 0.05). Transfection of the IFITM1 pcDNA3.1 construct decreased cell migration and invasion of HeLa cells, inhibited cell proliferation, and increased cell apoptosis.ConclusionIFITM1 gene expression may reduce the proliferation, migration, and invasion of cervical squamous cancer cells.
Highlights
Cervical cancer is a major cause of death in women worldwide
Interferon-induced transmembrane protein 1 (IFITM1), Ki‐67, and PCNA protein expression in cervical cancer and chronic cervicitis tissues measured by immunohistochemistry A total of 55 cervical cancer and 40 chronic cervicitis paraffin tissues were cut into triplicate sections to analyze the expression of IFITM1, Ki-67, and PCNA proteins
IFITM1 protein expression significantly decreased in cervical cancer tissues than in chronic cervicitis tissues (P < 0.01; Table 1)
Summary
Cervical cancer is a major cause of death in women worldwide. Interferon-induced transmembrane protein 1 (IFITM1) is involved in antivirus defense, cell adhesion, and carcinogenesis in different tissues. Cervical cancer is a major cause of death in women worldwide, with approximately 500,000 new cases and 280,000. Developed technologies, such as gene expression analysis, can be used to identify genetic alterations related to. We found that the mRNA expression level of the interferon-induced transmembrane gene (IFITM1) is reduced in cancer tissues [12]. Over 30 superfamily members of IFITM are involved in antivirus defense, immune cell signaling transduction, cell adhesion, carcinogenesis, and germ cell maturation [14]
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