Abstract
Objective To investigate the changes of myosin heavy chain (MyHC) isoforms in rat masseter muscle fibers caused by chronic sleep deprivation (CSD) and a possible link with the pathogenesis of temporomandibular joint disorders(TMD). Methods Total 180 male rats were randomly divided into three groups(n=60 per group): chronic sleep deprivation group (CSD), cage control group (CC), and large-platform control group (TC). Each group was further divided into three subgroups(n=20 in each group)according to the observation time point(7, 14, and 21 days). The expression of MyHC isoforms in masseter muscle fibers was investigated by real-time quantitative PCR, Western blotting and immunohistochemical staining. Results The expression of MyHC-Ⅰ, MyHC-ⅡA and MyHC-ⅡB deep and shallow masseter muscle in CSD7d group had differention with the control group(MyHC-Ⅰ: (0.314±0.005, 0.134±0.005, P 0.05), and there were no differences between the CC and TC groups at any time point. Conclusion These findings suggest that CSD alters the expression of MyHC isoforms, which may contribute to TMD pathogenesis. Key words: Chronic sleep deprivation; Temporomandibular joint disorders; Masseter muscle; Myosin heavy chain(MyHC)
Published Version
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