Effects of chlorobutanol on primary and secondary endings of isolated cat muscle spindles

Abstract

The effects of the preservative chlorobutanol on primary and secondary endings of muscle spindles isolated from the tenuissimus muscle of the cat were investigated in this study. Chlorobutanol was applied to the bathing solution in final concentrations of between 10 and 100 μg/ml. It induced a reversible and dose dependent decrease in the discharge frequency of both types of ending without any visible length change in the sensory region of the receptor. The initial activity, the peak dynamic discharge, the maximum static discharge value and the final static discharge value were evaluated from an ending's discharge pattern obtained during ramp-and-hold stretches. These four basic discharge frequencies decreased in parallel with increasing concentrations of chlorobutanol. Their sensitivities to chlorobutanol were similar (mean values: −0.11 to −0.29 imp/s per μg/ml chlorobutanol) and were independent of the amplitude of stretch. The dynamic response and the static response of both primary and secondary endings remained unchanged, indicating that the sensitivity of the spindle to stretch was not influenced by chlorobutanol. Chlorobutanol also reduced the discharge activity of the muscle spindle afferents during sinusoidal stretches. The amplitude of the receptor potential (AC component) remained unchanged under chlorobutanol. With the available recording technique it was not possible to measure slow shifts of the membrane potential. However, a hyperpolarization of the ending's membrane might explain why the afferent discharge frequency is reduced by chlorobutanol. The calcium dynamics of the spindle do not appear to be altered by CB, as the effect exerted on the afferent discharge by a change in the extracellular calcium concentration and a blockage of calcium channels was different from the CB effect. As the inhibitory effect of CB was reduced by ouabain, it is possible that CB activates the electrogenic Na/K pump or affects a mechanism that is closely related to the activity of the pump. The properties of the axonal membrane appear not to be altered, as chlorobutanol did not change the shape of action potentials.