Abstract
The researchers aimed to evaluate the functions of casein kinase 2 alpha subunit (CK2a) expression silencing in influencing pancreatic cancer (PC) PANC-1 cells from the aspects of invasion, proliferation and migration. As PC-1, PC PANC-1 and SW1990 cells exhibited significantly reduced CK2a mRNA expression level in contrast to normal pancreatic cells HPDE6-C7, while PC tissues displayed raised expression level compared with adjacent tissues (P<0.05). CK2a mRNA expression had significant correlations with nerve infiltration, lymph node metastasis, tumor differentiation degree and tumor-node-metastasis stage. After interfering with the expression of CK2a in PANC-1 cells, p-PTEN had elevated expression, while p-Akt473, p-GSK-3β, p-c-Raf, p-Akt308, p-PDK1, Snail and Vimentin, βcatenin had significantly down-regulated expressions, impeding cell growth, decreasing the number of healing wounds and invading cells. Moreover, the transplanted tumors were inhibited in terms of growth. For PC PANC-1 cells, their metastasis, proliferation and invasion were modulated by CK2a probably through regulating the PI3K/Akt/GSK-3β pathway that targeted epithelial-mesenchymal transition.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.