The effects of miRNAs-107 on cell proliferation, senescence and invasion of pancreatic cancer PANC-1 cells

Abstract

Objective To investigate the effects of miRNAs-107(miR-107) on pancreatic cancer proliferation, senescence and invasion. Methods MiR-107 expression levels in 3 pancreatic cancer cell lines PANC-1, ASPC-1, BXPC-3 and normal pancreatic HTERT-HPNE cells were studied by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). PANC-1 cells were transfected with 50 nmol/L anti-miR-107 or negative control using Lipofectamine 2000. After transfection, the miR-107 expression was measured by qRT-PCR. Cell proliferation was tested by methylthiazol tetrazolium (MTT) assay. Cell senescence was detected by β-galactosidase staining. The expression levels of PCNA, P16INK4A and MMP2 were measured by qRT-PCR. Results Compared with the HTERT-HPNE cells, the expression level of miR-107 in 3 pancreatic cancer cell lines was significantly increased (P<0.01). After transfected with 50 nmol/L anti-miR-107, cell proliferation was inhibited, and cell senescence were increased in PANC-1 cells (P<0.05), and there was no obvious change in cell invasion. Compared with the HTERT-HPNE cells, after transfected with anti-miR-107, the PCNA expression was significantly decreased and P16INK4A was significantly increased, but expression of MMP2 didn’t change significantly. Conclusions These results demonstrate that miR-107 promotes the proliferation and escapes cell senescence in PANC-1 cells by targeting PCNA and P16INK4A. But it has no obvious effects on cell invasion. Therefore, it may be a new target for the biologic therapy for pancreatic cancer. Key words: Pancreatic cancer; MiRNAs-107(MiR-107); Proliferation; Senescence; Invasion