Effects of Caffeine Intake on the Development of Deciduoma in Pseudopregnant and on Embryo Implantation in Pregnant Rats.

Abstract

Some epidemiologic studies have suggested that ingestion of caffeine increase the risk of spontaneous abortion, but most studies have used maternal self-reported caffeine consumption to estimate fetal exposure. This study was specially designed to test the direct effects of different doses of caffeine exposure on the decidualization of pseudopregnant and high doses used during implantation periods of pregnant rats. Uterine decidualization was induced bilaterally by twice of endometrial scratching on the morning of day 4 pseudopregnant rats. At the time of stimulation, one uterine horn installed histamine antagonist drug or different doses of caffeine; the contra lateral uterine horn was treated with saline solution as control. After 72 hours, the uterine deciduoma was significantly inhibited by histamine antagonist (p<0.05); contrast to histamine antagonist the caffeine-treated horn did not show the same inhibit results. We further estimated caffeine effect on the nature pregnant rats; they were feed with caffeine (60mg/kg/day) or saline by gastric instillation from gestation day 2 to gestation day7. At the end of this period, all of them were autopsied. There are no significant different in the total number of embryo counts or conceptus weight between these two groups. Our results indicate that caffeine has no direct effect on decidual formation in pseudopregnant rats and no direct influence on embryo implantation or conceptus weight in pregnant rats. Further work to confirm the distinct mechanisms of caffeine to influence early pregnant outcomes are required.