Effects of C5a anti-sense peptide on sepsis-induced abnormal coagulation and acute lung injury

Abstract

Objective To investigate the effects of C 5a anti-sense peptide on the pathogenesis of sepsis -induced abnormal coagulation and acute lung injury (ALI) in the early stage of sepsis. Methods The method of cecal ligature and puncture (CLP) was used to establish sepsis modeL Mice were randomly (random number) divided into control group, sham operation group, model group and C5a anti-sense peptide treatment group. In each group, pathological changes of the lung tissue and coagulation marker of mice were observed at intervals of 2 h, 4 h, 8 h and 12 hours after CLP. Meanwhile, the blood levels of tissue factor (TF) were detected by using ELISA at various intervals. Results The microscopicexamination showed that inflammatory cell infiltration, fibrin exudation and DIC occurred in lung tissue of mice in model group 2 hours after CLP, and those changes significantly lessened in mice of the treatment group. The levels of PT, TT, APTT and fragment D-dimer (DD) were significantly increased as time consumed, while fibrinogen (Fbg) and platelet (PLT) gradually and significantly decreased in model group and treatment group compared with control group and sham operation group ( P ＜ 0. 05 ), and those changes were greatly minimized in the treatment group than those in model group ( P ＜ 0. 05 ). The level of TF was (42.75±1.25) pg/mL in mice of control group and (60.73 ±1.43 ) pg/mL in mice of sham operation group. The level of TF of mice in model group gradually increased as time consumed to reach the highest level of ( 139. 81 ± 6. 71 ) pg/mL in 12 hours. Compared with model group, TF was ( 121.41 ± 7.21 )pg/mL in the intervention group (P ＜ 0. 05 ). Conclusions C5a anti-sense peptide can lessen the coagulation hyperactivity and ALL/ARDS symptoms in the early stage of sepsis. Key words: Animal; Sepsis; Acute lung injury; Acute respiratory dysfunction syndrome; Coagulation; Tissue factor; CSa Anti-sense peptide