Abstract
Fibroblast growth factors aFGF, bFGF, IGF-I, IGF-II, TGF-β, EGF, and PDGF did not stimulate the formation of stromal fibroblast colonies (CFC-F-colonies) in cultured murine adhesive bone marrow cells. It means that colony-stimulating activity of the bone marrow feeder cells with respect to the formation of stromal fibroblast colonies does not depend on the known growth factors. A scheme and conditions of culturing are developed for preparing conditioned media from bone marrow cell cultures which can replace the CFC-F colonystimulating activity of the bone marrow feeder cellsin vitro. Primary separation of conditioned media by ultrafiltration reveals that only the fraction with molecular weight of more than 65 kD exhibits CFC-F colony-stimulating activity.
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