Abstract
Objective To observe the effects of blue light on human RPE cells replicative senescence and expression of chemokines in RPE cell,and to discuss the role of blue light and inflammatory reaction in pathogenesis of age-related macular degeneration (AMD).Methods Healthy human eyeball was used for experiments.Primary human RPE culture was harvested with trypsin digestion.The cells were identified with immunohistochemistry.The blue light source of light emitting diode (2000±500)lux,(1000±200)lux,(500±100)lux were used to cause replicative senescence 1 hour a day,4 days of continuous irradiation and detect senescence associated beta-galactosidae (SA-β-Gal) activity.RT-PCR assay was used to detect expressions of chemokines IL-8,MCP-1 in RPE cells.Results This primary culture resulted in cells with well-preserved morphology using trypsin.The next passages were grown well.The number of the replicative senescence cells and the expressions chemokines IL-8,MCP-1 in RPE cells with blue particles increased as the increase of exposure intensity.The difference had statistics significance (P ＜0.05).Conclusions The replicative senescence of human RPE cells is positive correlation with blue light.The blue light increases the expressions of chemokins IL-8,MCP-1 in human RPE cells. Key words: Blue light; Retinal pigment epithelium; Replicative senescence; Inflammation; Chemotactic factor
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