Abstract
Background: VACUETTE® Blood Collection Tubes are used for the collection of venous blood. VACUETTE® tubes are used to collect, transport, store and process blood for testing serum, plasma or whole blood in the clinical laboratory for professional use. Objective: Evaluate the effect of blood specimen collection methods of the VACUETTE Serum Sep Clot Activator tube (gel tube), in determination vitamin A by HPLC. Methods: Blood specimen was divided into three parts, Three tubes, for Part I, No Additive tubes were drawn, for Part 2 Serum SepClot Activator tube (gel tube), and for Part 3 EDTA-coated plastic tubes. The tubes were gently mixed using ten complete inversions immediately following blood collection and centrifuged after venipuncture for 15 min and the supernatants were stored at -70°C until analysis. Vitamin A Testing was performed on healthy blood donors; the testing was performed by HPLC. Results: Our study showed that, There was no difference in the results were obtained with the serum (No Additive tube) and plasma tube in concentration of retinol 67.7 ug/dl and 73.8 ug/dl, but the results was 9.2 ug/dl obtained with the VACUETTE® Serum Sep Clot Activator tubes (gel tube). Conclusions: Tubes are drawn in a specific order to avoid the possibility of erroneous test results caused by carryover of an additive. If Serum tube-with or without clot activator or gel, it should be drawn as the first tube. We recommended that only serum and EDTA -plasma samples be used for Vitamin A determination by HPLC and cannot be carried out with all gel tubes.
Highlights
All VACUETTE® Serum Tubes are coated with micronised silica particles which activate clotting when tubes are gently inverted.VACUETTE® Serum Sep Tubes contain a barrier gel that is present in the bottom of the tube
Tubes are drawn in a specific order to avoid the possibility of erroneous test results caused by carryover of an additive
If Serum tube-with or without clot activator or gel, it should be drawn as the first tube
Summary
All VACUETTE® Serum Tubes are coated with micronised silica particles which activate clotting when tubes are gently inverted. VACUETTE® Serum Sep Tubes contain a barrier gel that is present in the bottom of the tube. Serum may be aspirated directly from the collection tube, eliminating the need for transfer to another container. Serum Clot Activator tubes are made of plastic and are used for the collection of venous blood, tubes should be used with care when measuring therapeutic drug levels because the drug/hormone may diffuse from the serum into the gel, causing a reduction in measured drug level [4,5,6,7]. VACUETTE® tubes are used to collect, transport, store and process blood for testing serum, plasma or whole blood in the clinical laboratory for professional use
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Chromatography & Separation Techniques
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
