Abstract
This investigation was designed to evaluate the role of Platelet-Rich Plasma (PRP) on healing of experimentally wounded skin in ten adult bucks, aged 2-3 years and weighing 25-30 kg. The animals divided randomly and equally into (control and treatment groups). Four of 3×3 cm of full-thickness square cutaneous wounds was induced on both sides of the lateral thoracic region of each animal under the effect of local anesthetic proceeding by xylazine hydrochloride as a sedative. A pair of left wounds was treated by injection with 5 mL of autonomous PRP (treatment group), 2 mm lateral to the wound edges and in the wound center. While, the right wound were injected by 5 mL of sterile saline by the same procedure (control group). Each group was divided into five subgroups (four wounds of each), for morph metrical and histopathological evaluations of wound healing process represented by percent of wound contraction, epithelialization and total healing at 3, 7, 14, 21 and 28 days post-wounding. The morphometrical appearance of the wounds which treated with PRP, showed that the contraction, re-epithelialization and healing percent were statically significant (p<0.05) in comparison with control wounds during four weeks study. Based on histopathological results, there was re-epithelialization of epidermis, with highly cellular granulation tissue, well differentiated keratinocytes of epidermis with scar formation in the dermis of the sectioned skin. We conclude that local injection of PRP leads to accelerate and improvement of wound healing in comparison to control wounds.
Highlights
The skin healing is the aim of studies and researches due to its clinical, scientific and economic interest, especially due to the great frequency of wounds caused by injury in goats
The wound's healing is a physiological phenomenon that initiates from the loss of integrity of the skin generating a solution of continuity that reaches the underlying layers in diverse degrees and depends on a series of chemical reactions classically divided in four phases: inflammation, a proliferative phase, remodeling and maturation (Kairuz et al, 2007)
The Plasma Rich in Growth Factors (PRGFs) is a mixture of autologous proteins concentrated from a determined volume of Platelet-Rich Plasma (PRP)
Summary
The skin healing is the aim of studies and researches due to its clinical, scientific and economic interest, especially due to the great frequency of wounds caused by injury in goats. The aim of wound care is to promote wound healing in the shortest time with minimal pain, discomfort and scarring to the patient and must occur in a physiologic environment conducive to tissue repair and regeneration (Priya et al, 2007). Despite numerous treatments available for deteriorated cutaneous wound healing, there is still the need for more effective therapy (Lee et al, 2011). The later stimulates angiogenesis, promoting vascular in growth and fibroblasts proliferation. It acts as hemostatic effect by forming a fibrin clot. A literature to assess the current clinical experience and the possible effects of (PRP) on wound-healing yields recorded by few reports (Kimura et al, 2005), the present study was designed to detect the role of PRP in healing of experimentally wounded skin in goats
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