Cytology of Bone Marrow Haematopoietic Cells of Village Weavers (&lt;em&gt;Ploceus cucullatus&lt;/em&gt;)

A.J Jubril,A.T Adetuga,T Omadevuaye,A.O Omonona

doi:10.19026/ijava.9.5922

Abstract

In order to study the cellular morphology and Myeloid/Erythroid (M/E) ratio in the bone marrow of the adult village weaver birds ( Ploceus cucullatus ), bone marrow samples were collected from the proximal tibiotarsus bone of 30 clinically-healthy adult village weaver birds during post mortem. The bone marrow smears were fixed and stained using the Romanowsky Giemsa Stain (RGS), then viewed microscopically. Blood samples were also collected ante mortem for routine haematological examination. The results showed that the mean M/E ratio was 0.19, the mean erythroid cell percentage was 72.93, the mean myeloid cell percentage was 19.8 and the mean percentage of all other cells was 0.33. The Myeloid/Erythroid (M/E) ratio obtained from this study is comparable to that of quails. The morphology and distribution of blood cells in the bone marrow of adult village weaver birds were similar with reports from other birds such as ducks and black-head gull. Other findings from this study are comparable to existing data in the literature. The peripheral blood evaluation was within reference ranges. There is need to further determine the mean sizes of all cells of the bone marrow of village weaver birds and compare with that of other birds.

Highlights

Haematopoiesis occurs primarily in the bone marrow of post-hatch birds, haematopoeitc activity may be found in various internal organs like liver and possibly spleen (Kim, 2010; Lopez et al, 2014)
Bone marrow aspirates were taken from 30 clinically-healthy adult village weaver birds with an average weight of 32 g captured from roosting sites within Ibadan Metropolis, Nigeria
The cellular composition of the bone marrow of village weaver birds showed that the mean percentage for erythroid and myeloid cells were 72.93 and 19.8 respectively

Summary

Introduction

Haematopoiesis occurs primarily in the bone marrow of post-hatch birds, haematopoeitc activity may be found in various internal organs like liver and possibly spleen (Kim, 2010; Lopez et al, 2014). The bone marrow is the definitive hematopoietic tissue containing the pluripotent hematopoietic stem cells that differentiates along all the blood cell lineages. It is found within the deepest depths of bones filling the space between the bone and the trabecular beams (Dean, 2005; Kulikov et al, 2014). The bone marrow in birds is localized mainly in the peripheral skeleton and about 45% in the humeral, femoral and tibia bones, whereas its quantity is very small in the axial skeleton or is sometimes not present (Almany Magal et al, 2014). This is contrary to what is obtainable in mammals where the main mass of the bone marrow is found in the axial skeleton (Farhi, 2009)

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