Effects of Antimetabolites and Physical Modification on Endosteal and Endothelial Zeta-Potentials

Abstract

The endosteal surface of long bones of dogs prepared for streaming potential measurements was shown by Feulgen and PAS reactions to be heterogenous in composition. FDA-PI treatment showed that most of the cells remaining on the endosteal surface were living. Streaming potentials of the endosteal surface of bones and the luminal surface of arteries of dogs were measured in 0.01 M HEPES, pH 7.4 in 5.2% glucose before and after treatment with KCN, DNP, glutaraldehyde, or heparin, and used to calculate ξ-potentials. KCN, DNP and glutaraldehyde significantly altered ξ-potentials of bones whereas only DNP and heparin significantly changed the ξ-potentials of arteries. When the relative amount of endosteal surface of bone composed of exposed bone matrix was increased by drilling the ξ-potentials also increased. The results indicate that: a) both cellular and non-cellular elements contribute to the surface charge of endosteal bone, and b) the heterogenous endosteal bone surface and the cellular luminal arterial s...