Effects of andrographolide and budesonide on the expressions of interleukin-17 and interleukin-23 in asthmatic mice model

Abstract

Objective To evaluate the effects of andrographolide and budesonide (BUD) on eosinophil(EOS) counts and neutrophilic and the expressions of IL-17, IL-23 in asthmatic mice. Methods BALB/c mice were randomly divided into asthmatic group, andrographolide group, BUD group and control group, 8 mice in each group.The mice were sensitized and challenged by ovalbumin(OVA) to establish asthmatic mice model.Two groups of mice were treated with andrographolide and BUD, respectively.Twenty-four hours after the last administration, inflammatory cell count, cell classification in bronchoalveolar lavage fluid (BALF) and lung histological examination were carried out.Wright-Giemsa was used to count total, EOS, neutrophilic cells of BALF.Meanwhile, ELISA was used to detect the concentrations of IL-17 and IL-23 of BALF and peripheral blood.The expressions of IL-17 mRNA and IL-23 mRNA of the lung tissue were detected by quantitive Real-time PCR. Results Total cell and EOS counts in BALF of 2 treatment groups[(111.98±3.28)×107/L and (64.16±36.75)×107/L, (131.29±4.89)×107/L and (82.40±11.48)×107/L] decreased significantly compared with asthma group[(208.78±18.52)×107/L and (106.80±13.90)×107/L] (all P 0.05). IL-23 protein levels in BALF of BUD treated group[89.92 (113.87-67.79) ng/L] were significantly lower than asthma group[93.30 (173.39-42.95) ng/L] (P 0.05). Conclusions Andrographolide and BUD can suppress the infiltration of total inflammatory cells and EOS expression IL-23 mRNA in BALF.They had no significant impact on the neutrophilic cells counts and expression of IL-17 in BALF. Key words: Asthmatic mice model; Andrographolide; Budesonide; Interleukin-17; Interleukin-23