Abstract
To investigate the impact of AGEs with different concentrations and different times on the expression of UⅡ and GPR14 mRNA in MC. Methods In vitro rat mesangial cells, by adding different concentrations of AGEs(final concentration0、25、50、100和200 mg/L)，37°C incubated for 24 h,AGE100 mg/L groups were cultured 0、2、8、16 and 24 h separately，established the control groups respectively, collecting MC,extracted total cellular RNA for RT-PCR reaction and detected the expression of UⅡ and GPR14 mRNA in MC. Results The expression of UⅡ and GPR14 mRNA in MC were increased with the change of AGEs concentrations from low to high.Compared with AGEs 0 mg / L group, the expression of UⅡ and GPR14 mRNA in AGEs50, 100, and 200 mg / L groups were significantly higher (P<0.05); In AGEs100mg / L groups, the expression of UⅡ and GPR14 mRNA in MC were increased with the time extended, compared with AGEs 0h group, the expression of UⅡ and GPR14 mRNA in AGEs8, 16, and 24h groups were significantly higher (P<0.05); There were no change in control groups（P＞0.05）. Conclusion AGEs can significantly increase the expression of UⅡ and GPR14 mRNA in MC.
Published Version
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