Effects of Administration of Alpha-Melanocyte Stimulating Hormone (α-MSH) on Some Hematological Values of Alloxan-induced Diabetic Rats

Kamal Mohmoud Saleh Mansi

doi:10.3844/ajptsp.2006.5.10

Abstract

This study was designed to evaluate the role of alpha- melanocyte stimulating hormone (α-MSH) on heart rate and some hematological values in alloxan induced diabetic rats. 40 male white rats were divided into four experimental groups: control, diabetic, α-MSH-treated and α-MSH-treated diabetic. At the end of the experimental period (3weeks), animals in all four groups were fasted for 12 hrs and blood samples were taken for the determination of plasma insulin, Glucagon, glucose levels, RBC and WBC (red and white blood cell) counts, packed cell volume (PCV), erythrocyte sedimentation rate (ESR) and hemoglobin (Hb) concentration. It was found that (α-MSH) increased the lowered RBC and WBC counts, PCV and neutrophil percentage in diabetic rats. However the WBC counts of the (α-MSH) treated diabetic group was still lower than the control and (α-MSH) group. (α-MSH) also decreased the elevated heart rate, ESR and glucose concentration of diabetic rats. The hormone was investigated for hypoglycemic effect in diabetic rats and induced not significant reduction in serum glucose from (19.83±1.25 MmolL) in diabetic group to (15.7±1.10 MmolL) in (α-MSH)-treated diabetic group. However the blood glucose still higher than the control and (α-MSH) group, serum insulin increased from (0.55±0.08 ng mL-1) in control group to (0.65±0.06 ng mL-1) in α-MSH-treated group and still higher than control in α-MSH-treated diabetic (0.59±042 ng mL-1) and serum Glucagon increased in diabetic, α-MSH-treated and α-MSH-treated diabetic groups. It is concluded that treatment (α-MSH) might decrease the diabetes-induced disturbances of heart rate and some hematological parameters of alloxan-induced diabetic rats.

Highlights

Diabetes Mellitus (DM) represents a heterogeneous group of disorders that have hyperglycemia as a common feature[1,2]
Great changes in the human environment, behavior and lifestyle resulted in the raising rates of Diabetes[3]. (DM) is consequence of defects in secretion, insulin action or both, which is translated into abnormalities of carbohydrate, fat and protein metabolism resulting in hyperglycemia[4,5]
As shown in Insulin level decreased in diabetic rats (0.38±0.08 ng mL 1) and still higher than control in -MSH) group. ( (MSH)-treated diabetic (0.59±042 ng mL 1) (p< 0.05) (Table 3) and serum Glucagon increased in diabetic (64±0.58 pg mol 1) (p< 0.05), -MSH-treated (38±0.40 pg mol 1) and -MSHtreated diabetic groups (58±0.21 pg mol 1) (p< 0.05) compared to control (32±2.23 pg mol 1)

Summary

Introduction

Diabetes Mellitus (DM) represents a heterogeneous group of disorders that have hyperglycemia as a common feature[1,2]. - MSH is known to have pleitrophic functions including pigmentary, anti-inflammatory, antipyretic and immunoregulatory roles in the mammalian body as reduction nterleukin-1 beta effects on rat stomach[17], inhibits lipolyscchride-induced biological responses by down regulating CD14 from macrophage, suppresses antigen-stimulated T-cell production[18]. Haematological analysis: At the end of the experimental period (3 weeks) the animals in all groups were fasted for 12 hrs and blood samples were taken to determine glucose levels, RBC, WBC counts, packed cell volume (PCV), erythrocyte sedimentation rate (ESR) and haemoglobin (Hb) concentration.

Results

Conclusion