Abstract
Objective To investigate the effects of adenovirus transfected with IκB (Ad-IκB) on multiple organ dysfunction syndrome (MODS) induced with oleie acid and lipopolysaccharide (L P S) in rats. Methods Fifty pathogen-free SD rats of both sexes weighing 100-120 g were randomly divided into 3 groups: group Ⅰ control (C,n = 10); group Ⅱ MODS (M, n = 20) and group Ⅲ Ad-IκB + MODS (T, n = 20). The animals were anesthetized with intraperitoneal (IP) chloral hydrate 0.3-0.4 ml/100 g. MODS was induced with iv oleic acid 0.25 ml/kg and iv LPS 3.5 mg/kg at an interval of 4 h in group M and T. In group T (Ⅲ) Ad-h:B was given iv simultaneously with LPS. Blood samples were taken from abdominal aorta at 1 and 7 days after LPS or IκB transfected administration for determination of plasma creatine phosphokinase, glutamic pyruvie transaminase (GPT), total bilirubin and blood creatinine and blood gas analysis. The animals were then sacrificed and the liver and lungs were removed at 1 and 7 days after blood samples were obtained for determination of expression of NF-κB p65 in lung and liver tissue using immuno-histochemical staining and Western blotting, Immuno-histochemical staining was scored (0 = no positive cells, 4 = 81%-100% positive cells). Results Creatinine, GPT, total bilirubin and creatine phosphokinase were significantly higher in group M than in group C and IκB transfected significantly attenuated the MODS-induced increase. PaO2 at day 7 was significantly lower in group M and T than in group C and was signilicantly higher in group T than in group M. The expression of NF-κB p65 protein was significantly higher in group M than in group C and was significantly decreased by IκB transfected in group T. The immuno-histochemical staining scores were highest in group M and lowest in group C. Conclusion Ad- boB treatment can inhibit the activation of NF-κB and synthesis of inflammatory factors, resulting in amelioration of MODS induced with oleic acid and LPS. Key words: Transfection; Multiple organ dysfunction syndrome; Oleic acid; Endotoxins; NF-κB
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
