Effects of acute and chronic chromium stress on the expression of heat shock protein genes and activities of antioxidant enzymes in larvae of Orthetrum albistylum

Abstract

The dragonfly species Orthetrum albistylum, can accumulate heavy metals from its aquatic environment and thus serves as a biological indicator for monitoring and evaluating water quality. Heat shock proteins (HSPs) play important biological roles in resistance to various types of environmental stress. The full-length cDNA sequences of the heat shock cognate (hsc) 70 and heat shock protein (hsp) 70 genes were cloned from O. albistylum larvae. Relative levels of expression of hsc70 and hsp70 in the head, epidermis, midgut, and adipose tissue were measured by qRT-PCR after chronic and acute contamination of 5–8 instar larvae with chromium (Cr) solution, and under control conditions. Activities of superoxide dismutase (SOD) and catalase (CAT) in chronically contaminated larvae were also measured. Phylogenetic analysis revealed that the cloned hsc70 and hsp70 genes were highly homologous to known HSP70 family members reported in other insects. The mRNA levels of hsc70 and hsp70 did not differ significantly in various larval tissues. Under chronic chromium stress, hsc70 and hsp70 expression were upregulated to a maximum and then downregulated; hsp70 mRNA levels were higher than those of hsc70 at all concentrations of chromium. Under acute chromium stress, hsc70 expression was inhibited at low chromium concentrations and upregulated at chromium concentrations higher than 125 mg/L. However, hsp70 expression was higher than that in the control group and markedly higher than that of hsc70. Changes in SOD and CAT activities displayed consistent trends for different chronic chromium concentrations, first increasing and then decreasing over time. Collectively, these findings demonstrated the response of the HSP family of genes and antioxidant enzymes following exposure to heavy metal stress, as well as their potential applicability as biomarkers for monitoring environmental pollutants.