Abstract
ObjectiveThis study aimed to explore the effects of activating GABAB1 receptor by baclofen on proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of ovarian cancer cells.ResultsOne hundred μmol/L, 200 μmol/L and 300 μmol/L were selected as low, medium and high baclofen concentrations respectively. Cells were divided into four groups: Control, 100 μmol/L, 200 μmol/L and 300 μmol/L. Compared with the control group, the viability, colony formation, migration and invasion of SKOV3 cells were inhibited, and the apoptosis of SKOV3 cells were enhanced significantly at 200 μmol/L and 300 μmol/L baclofen. Moreover, they changed significantly with the increase of baclofen concentration. Compared with the control group, the expression of E-cadherin and GABAB1 increased and the N-cadherin expression decreased significantly in 200 μmol/L and 300 μmol/L groups. Higher concentration of baclofen induced higher expression of E-cadherin and lower expression of N-cadherin.ConclusionBaclofen inhibited the proliferation, cloning, migration, invasion and EMT of ovarian cancer cells by activating GABAB1 receptor. These results might contribute a lot to clarify the role and possible mechanism of GABAB1 receptor in ovarian cancer.
Highlights
Ovarian cancer is the third most common malignant tumor in female reproductive system, and it has the highest mortality rate among gynecological tumors [1]
In this study, we aimed to explore the effects of activating GABAB1 receptor by baclofen on the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of SKOV3 cells, so as to clarify the role and possible mechanism of GABAB1 receptor in ovarian cancer
This study might provide a theoretical basis for the further development of anti-cancer drugs coupled with GABAB1 receptor
Summary
Ovarian cancer is the third most common malignant tumor in female reproductive system, and it has the highest mortality rate among gynecological tumors [1]. Ovarian cancer is featured with hidden onset, difficulty in early detection, high degree of malignancy and easy recurrence and metastasis [1]. Epithelial tumors are the most common among all primary ovarian cancer, accounting for about 50–70% [2]. Due to the low response rate to drug therapy and poor prognosis, the mortality rate of epithelial ovarian cancer is still high [3,4,5]. Intensive study of epithelial ovarian cancer is of great economic and social significance for the early detection and treatment of ovarian cancer. The development and progression of ovarian cancer is a multi-factor and complex process, where multi-gene and
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