Abstract
Aminoglutethimide [α- (p-aminophenyl)- α-ethyl-glutarimide], an inhibitor of cholesterol side chain cleavage, largely reverses the inhibitory effects of ACTH and adenosine- 3′,5′-monophosphate (CAMP) on in vitro incorporation of 3H-leucine and 14C-orotate into rat adrenal protein and RNA, respectively. With aminoglutethimide present, small stimulatory effects of ACTH and CAMP on leucine incorporation are apparent, and these effects are not blocked by actinomycin D. Small stimulatory effects of ACTH on orotate incorporation are also apparent in the presence of aminoglutethimide, but CAMP produces a biphasic response with inhibition and reversal of inhibition at lower and higher CAMP concentrations, respectively. The inhibitory effect of CAMP may be due to conversion to 5′-AMP since the latter is purely inhibitory and since caffeine blocks CAMP-induced inhibition of orotate incorporation. (Endocrinology85: 1209, 1969)
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