Abstract
Summary Two cardenolide accumulating, highly differentiated Digitalis lanata tissue culture strains were treated with abscisic acid (ABA) and gibberellic acid (GA 3 ) (both 1 μM). Strain S-1 was grown in light (S-1L) or darkness (S-1D), and the light sensitive strain S-2 was grown in darkness only (S-2D). The negative effects of ABA on growth and cardenolide accumulation were eliminated by GA 3 in strain S-2. Growth of strain S-1 in darkness, which was stimulated by GA 3 , was further increased when ABA was added. For strain S-1 in light, ABA was negative for both growth and cardenolide accumulation, while GA 3 was positive for growth and negative for cardenolide accumulation. Tissue differentiation was stimulated by GA 3 in S-1L and S-2D. This stimulation was decreased when ABA was also added. The pH in the culture medium at harvest was lower in strain S-2 than in strain S-1.
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