Effects of a combination of Sauropus androgynus L. leaf and Zingiber Ottensii rhizome on fatty acid profile and liver damage in rats

Abstract

Background: High fat and carbohydrate diets may lead to the malfunction of hepatic cells because of excessive lipolysis of fat tissues and the subsequent increase of free fatty acids in the liver. This is characterised by histological changes in the hepatic cells which then undergo necrosis. High fat and carbohydrate diets may also lead to gut microbiota dysbiosis. Objectives: This study aims to determine the effect of the combination of katuk leaf and black bangle rhizome on the short chain fatty acid (SCFA) profile and liver cell damage in rats induced by a high fat and carbohydrate diet. Methods: The research method used is a preventive experimental study in vivo. Four groups of test animals were distinguished into negative group, positive group, comparative group, and 15% combination test group, which was carried out for 21 days. Examined parameters were: SGPT (Serum Glutamic Pyruvic Transaminase), SGOT (Serum Glutamic Oxaloacetic Transaminase), Triglyceride, and hepatic cells histopathology. The results were examined by SPSS and showed a significant difference (p < 0.05). Results: Parameter results showed the reduction of SGOT in the combination test group. The combination of katuk leaf and black bangle rhizome can increase acetic acid and lower the Manja Roenigk score, and it is possible to inhibit liver cell damage. Conclusion: The combination of katuk leaf (Sauropusxandrogynus L.Merr) and black bangle rhizome (Zingiber ottensii Val) may affect the SCFA levels and help lower the risk of liver cell damage.