Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis

Nigar Saeed,Sabahattin Cömertpay

doi:10.18016/ksutarimdoga.vi.627056

Abstract

Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production quite challenging. In this study, effects of three concentrations of 6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F. imperialis plantlets in vitro were determined. The auxins were at similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all treatments. It has been found that the size and number of bulblets, as well as root and shoot numbers in regenerated plantlets were increased significantly (p&lt;0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10 mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots in all doses studied (p&lt;0.05). The supreme concentration of BAP (0.15 mg/L) decreased the bulblet number in all treatments, however, when supplemented with 3.5 g/L of AC, it was found to raise all the values measured (p&lt;0.05). Overall, the best growth of the plant parts from callus were achieved with the highest concentration of AC and 1.5 mg/L of BAP.

Highlights

Fritillaria imperialis L. belongs to Liliaceae family, and. it can be used as ornamental plant and in flower arrangements
We evaluated the impact of three different doses (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) by itself or in combination with three different amounts (0.00, 0.10, 0.15 mg/L) of BAP on the growth of the plantlets of F. imperialis, cultured via indirect organogenesis
When we made the first measurements on the 9th week, we found that the moderate amount (0.10 mg/L) of BAP along with the maximum dose of AC (3.5 g/L) produced more bulblets, roots and shoots (13.00±3.1, 23.40±5.6 and 16.60±1.9) compared to the control group (1.20±0.4; 14.60±2.0; 14.20±3.30) to which no AC and no BAP was applied (Table 1)

Summary

Introduction

Fritillaria imperialis L. belongs to Liliaceae family, and. it can be used as ornamental plant and in flower arrangements. Under in vitro culture conditions, the growth of the bulblets of F. imperialis can be stimulated by a combination of plant growth hormones (Li et al, 2011).

Results

Conclusion