Effects of 12 Weeks of Hypertrophy Resistance Exercise Training Combined with Collagen Peptide Supplementation on the Skeletal Muscle Proteome in Recreationally Active Men.

Vanessa Oertzen-Hagemann,Marius Kirmse,Katrin Marcus,Kathy Pfeiffer,Petra Platen,Britta Eggers,Markus De Marées

doi:10.3390/nu11051072

Abstract

Evidence has shown that protein supplementation following resistance exercise training (RET) helps to further enhance muscle mass and strength. Studies have demonstrated that collagen peptides containing mostly non-essential amino acids increase fat-free mass (FFM) and strength in sarcopenic men. The aim of this study was to investigate whether collagen peptide supplementation in combination with RET influences the protein composition of skeletal muscle. Twenty-five young men (age: 24.2 ± 2.6 years, body mass (BM): 79.6 ± 5.6 kg, height: 185.0 ± 5.0 cm, fat mass (FM): 11.5% ± 3.4%) completed body composition and strength measurements and vastus lateralis biopsies were taken before and after a 12-week training intervention. In a double-blind, randomized design, subjects consumed either 15 g of specific collagen peptides (COL) or a non-caloric placebo (PLA) every day within 60 min after their training session. A full-body hypertrophy workout was completed three times per week and included four exercises using barbells. Muscle proteome analysis was performed by liquid chromatography tandem mass spectrometry (LC-MS/MS). BM and FFM increased significantly in COL compared with PLA, whereas no differences in FM were detected between the two groups. Both groups improved in strength levels, with a slightly higher increase in COL compared with PLA. In COL, 221 higher abundant proteins were identified. In contrast, only 44 proteins were of higher abundance in PLA. In contrast to PLA, the upregulated proteins in COL were mostly associated with the protein metabolism of the contractile fibers. In conclusion, the use of RET in combination with collagen peptide supplementation results in a more pronounced increase in BM, FFM, and muscle strength than RET alone. More proteins were upregulated in the COL intervention most of which were associated with contractile fibers.

Highlights

Skeletal muscle is a dynamic tissue that adapts to external conditions and reacts to acute and long-term physical activity and feeding conditions [1]
The underlying mechanism of adaptation is an increase in muscle protein synthesis (MPS) stimulated through mechanical load, which can be further augmented by additive protein
Post-hoc t-tests with the Bonferroni correction revealed no significant differences between groups in body mass (BM), fat-free mass (FFM), and R after 12 weeks

Summary

Introduction

Skeletal muscle is a dynamic tissue that adapts to external conditions and reacts to acute and long-term physical activity and feeding conditions [1]. Maintenance of muscle mass is important for athletes, and for health and aging [2]. Resistance training using a repeated high-weight stimulus optimizes the preservation of muscle mass that is correlated with body strength [3]. The underlying mechanism of adaptation is an increase in muscle protein synthesis (MPS) stimulated through mechanical load, which can be further augmented by additive protein. Amino acids are required for protein synthesis. A branched-chain amino acid (BCAA), has been shown to be a prerequisite stimulator of the mammalian target of rapamycin (mTOR) signaling pathway, which plays a critical role in MPS [5,6]

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