Effects of 1-Methylcyclopropene (1-MCP) and Ethylene on Postharvest Lignification of Common Beans (Phaseolus vulgaris L).

Abstract

Postharvest 1-methylcyclopropene (1-MCP) treatment can inhibit the lignification of fruits and vegetables. The mode of action of 1-MCP is through inhibiting ethylene production, but the effect of 1-MCP and ethylene on lignification of common beans remains unknown. This work compared the effect of 0.5 μL L–1 1-MCP and 100 μL L–1 ethylene on the lignification of common beans during storage. Postharvest 1-MCP significantly inhibited the increase of the lignified cell group, sclerenchyma became thicker, vascular bundles thickened, and lignified cells grew during storage, while ethylene was the opposite. 1-MCP inhibited the increase in the respiration rate, sucrose phosphate synthase (SPS), sucrose synthase (SuSy), phenylalanine ammonialyase (PAL), cinnamyl alcohol dehydrogenase (CAD), and peroxidase (POD), whereas ethylene increased all of them. Ethylene treatment stimulated and 1-MCP inhibited the decline of reducing sugar and cellulose content. Expression of genes, including PvACO1, PvAOG1, PvSuSy2, PvPAL3, Pv4CL1, and PvCOMT1, with the lignin content being significantly increased in common beans during storage. 1-MCP treatment markedly inhibited the expression of PvACO1, PvSuSy2, PvPAL3, Pv4CL1, and PvCOMT1 genes, while strengthened the expression of PvETR1 and PvAOG1, while ethylene was the opposite. This work provides evidence that ethylene or abscisic acid (ABA) may play an important role in 1-MCP regulation of postharvest lignification in common beans and provides strategies for preserving the quality of fruits and vegetables during storage.