Abstract
Senggani leaves are one of the highland plants in Indonesia with the latin name Melastoma candidum. The results of phyitochemical screening that have been carried out show that senggani leaves contain tannin and flavonoid hich have an antibacterial function by inhibiting bacterial action in cell formation and even growth. The survey metode used is descriptive (for explanation of plants) and experimental (for an explanation of antibacterial test). In the bacterial test using a concentration of leaf extract of 6,25%, 12,5%, 25%, 50%, 75% and 100%. An placebo as positive control (+) and DMSO as a negative control (-). Antibacterial to streptococcus mutans (gram-positive bacteria) which at concentration of 6,25% has an average 12 mm, 12,5% has an average 13,56 mm, 25% has an average 15,30 mm, 50% on average 17,20 mm, 75% on average 18,10 mm and 100% have an average of 19,06 mm. So, senggani leaves have effect antibacterial to Streptoccous mutans.
Highlights
Pendahuluan Indonesia memiliki berbagai jenis tanaman yang banyak memiliki manfaat, faktor tersebut karena di Indonesia memiliki iklim yang cocok dan tanah yang subur
The results of phyitochemical screening that have been carried out show that senggani leaves contain tannin and flavonoid hich have an antibacterial function by inhibiting bacterial action in cell formation and even growth
In the bacterial test using a concentration of leaf extract of 6.25%, 12.5%, 25%, 50%, 75% and 100%
Summary
Satu koloni bakteri Streptococcus mutans diambil dengan menggunakan jarum ose steril. Koloni bakteri tersebut kemudian ditanamkan pada media nutrient agar miring dengan cara menggores, setelah itu diinkubasi dalam inkubator pada suhu 370C selama 18-24 jam. Peremajaan Bakteri Satu koloni bakteri/jamur diambil dengan jarum ose steril, lalu ditanam pada media NA miring dengan cara menggores. Kemudian diinkubasi dalam inkubator pada suhu 370C selama 18-24 jam. Koloni bakteri bakteri Streptococcus mutans diambil dari stok kultur dengan jarum ose steril, lalu disuspensikan dalam tabung reaksi yang berisi 10 ml larutan nutrient broth diinkubasi selama 18-24 jam. Uji Aktivitas Antibakteri Sebanyak 0,1 ml dari inokulum dimasukkan dalam cawan petri steril, kemudian dituang media NA sebanyak 15. Diletakkan cakram kertas yang telah direndam pada setiap konsentrasi dan dibiarkan 15 menit, kemudian diinkubasi pada inkubator suhu 370C
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