Effectiveness of Sodium Hypochlorite plus EDTA Compared with Peracetic Acid in Removing Smear Layer and Killing Enterococcus faecalis.

Abstract

The aim of this study was to evaluate the effectiveness of 2.5% sodium hypochlorite associated with 17% Ethylenediaminetetraacetic acid (NaOCl-EDTA), versus that of 1% peracetic acid (PA), in removing the smear layer, as assessed by scanning electron microscopy (SEM), and in exerting bactericidal action against Enterococcus faecalis (E. faecalis ) , as assessed by the real-time polymerase chain reaction (real-time PCR). Fifty-five extracted mandibular single-rooted premolars were selected, and divided into two experimental groups (NaOCl-EDTA and PA; n=25) and one control group (0.9% saline; n=5). Pre- and post-instrumentation samples were collected and assessed for the presence of E. faecalis using real-time PCR. The teeth were instrumented using hand files and the ProTaper Universal system (hybrid technique) for a standardized time of 7 min. A total of 20mL of NaOCl followed by 5mL of EDTA were applied during instrumentation in the NaOCl-EDTA group, whereas 20 mL of PA and 20 mL of saline were applied in the PA and control groups, respectively. An additional 5 mL of saline was applied in all the groups to neutralize the environment. A scoring system was used to conduct the SEM assessment. The results were submitted to the Kruskal-Wallis test, complemented by Dunn's test (SEM analysis) (P<0.05). A significant microbial reduction was observed in both the PA and the NaOCl-EDTA groups (P<0.05). In the PA group, the presence of a smear layer in the apical third was significantly greater than in the cervical third (P<0.05); no significant differences were observed between the middle and cervical thirds, or between the middle and apical thirds (P>0.05). In the NaOCl-EDTA group, the smear layer scores were significantly higher in the apical third than in the cervical and middle thirds (P<0.05). This in vitro study showed that there was no significant difference between PA and NaOCl-EDTA irrigation regimens regarding either antimicrobial action against E. faecalis or removal of the smear layer, except for greater removal in the middle third by the NaOCl-EDTA group.