Abstract
Many kinds of pesticides have been developed and used to yield a good harvest but the residues in agricul-tural products cause health problems. It is important to keep watch on these residues by using adequate methods of analysis. Pretreatment such as gel permeation chromatography (GPC) or column chromatography is often needed for the quantitative analysis of pesticide in agricultural products by conventional methods such as gas chromatography/low resolving power mass spectrometry (GC/LRMS). However, these pretreat-ments need a lot of work and take time. New methods saving the necessity of these pretreatments have been desired. We have applied selected reaction monitoring (SRM) to quantitatively determine cypermethrin residues in Perilla frutescens samples and compared the results with LRMS and HRMS in SIM mode. A background peak caused by the matrix overlapped the cypermethrin peak in the analysis using LRMS. SRM and HRMS in SIM mode provided chromatograms without matrix interference. The high selectivity of the product ion (m/z 127) produced from precursor ion (m/z 163) isolated the target peaks from the matrix peaks when using SRM. This method eliminates the pretreatment step, thus saving time and simplify ing the ana-lytical process.
Highlights
Many kinds of pesticides have been used for the control of harmful insects in agriculture
It is important to keep watch on these residues by using adequate methods of analysis. Pretreatment such as gel permeation chromatography (GPC) or column chromatography is often needed for the quantitative analysis of pesticide in agricultural products by conventional methods such as gas chromatography/low resolving power mass spectrometry (GC/LRMS)
Four peaks attributed to the isomers of cypermethrin were detected at retention times of 30.87, 31.06, 31.20 and 31.28 min
Summary
Many kinds of pesticides have been used for the control of harmful insects in agriculture. The legal limit for cypermethrin in Perilla frutescens leaves was set to be less than 6 g/ml This underscores the need for efficient analytical procedures for pesticide residue detection in agricultural products. High resolving power mass spectrometry (HRMS) and selected reaction monitoring (SRM) were applied. These methods can isolate target peaks from matrix peaks saving pretreatments. Using these methods, we analyzed cypermethrin added intentionally to the extracts of the leaves of Perilla frutescens and the results were compared with that obtained by using LRMS
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