Effectiveness of Nanosilver Biosynthesis using Inulin Gembili Tuber (Dioscorea esculenta L.) on Variation of Inulin Solution Towards Particle Sizes and Antibacterial Activities

Abstract

Inulin is one of fiber and carbohydrates sources derived from plants. In the medical fields, inulin is used for prebiotic and antibacterial agent. Inulin is generally supplied by importing from America and England. Gembili is an Indonesia local tuber that is reported to have potential as a high source of inulin. In this study, the biosynthesis process was carried out using silver nitrate to produce a better properties and more effective than starting material. This study aims to determine the effectiveness of biosynthesis nanosilver using inulin gembili in variational solubility temperature of inulin at 25 °C and 60 °C to produce the expected particle size and antibacterial activity against S. aureus and E. coli bacterias. The results of this study are biosynthesis nanosilver using inulin gembili that dissolved at temperature of 25 °C produce particle size of 29.8 nm, whereas at temperature of 60 °C produces 35.4 nm. Meanwhile, variations in temperature of inulin solubility did not show any significant difference towards silver nitrate control in the antibacterial activity test against S.aureus and E.coli, even though the FTIR spectra showed that interaction between functional groups of silver nitrate and inulin gembili. Therefore, in this study, inulin function as a capping agent and did not induce the antibacterial activity of silver nitrate.