Abstract
Abstract: The aim of the study was to determine the antibacterial activity of buni skin-pulp ethanolic extract against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. Profile of antibiotic resistance which is growing among Staphylococcus aureus and Escherichia coli need some exploration of antibacterial activity buni skin-pulp because the contain of anthocyanin which has antibacterial activity. The research was purely experimental research with randomized complete direct sampling design. The extraction method was done by macerated in ethanol solvent. Tube tests and Thin Layer Chromatography (TLC) were used to determine the content of the secondary metabolites substance in buni skin-pulp ethanolic extract. Antibacterial activity test was done by diffusion method, then followed with liquid dilution method to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The antibacterial activity was evaluated based on the result of inhibition zone diameter then analyzed with Kruskal-Wallis test followed with Mann-Whitney test. The results showed that the chemical substances of the buni skin-pulp ethanolic extract predicted with TLC assay were phenolic, flavonoids, and anthocyanin compounds. The antibacterial activity showed that the ethanolic extract only had antibacterial activity against Staphylococcus aureus ATCC 25923 with MIC and MBC values 30% and 33%, respectively. Keywords: antibacterial potency, buni skin-pulp, Staphylococcus aureus, Escherichia coli
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