Abstract
Gut microbiota is very important for energy metabolism and regulation, which in turn affect the health and physiological functions of the host, and provide energy required for exercise. Supplementation with probiotics may be one of the ways to change the gut microbiota. In recent years, many studies have shown that probiotic supplementation can effectively improve sports performance. In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition. Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks. The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage. Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05). We believe that PL-02 can be used as a supplement to improve exercise performance and for its anti-fatigue effect.
Highlights
Exercise-induced fatigue can be roughly divided into central fatigue and peripheral fatigue, which is a common and complex multi-dimensional s ymptom[1]
Probiotics have been commonly used to promote health and improve body function, as well as for different benefits depending on the characteristics of the strain
We screened the human-origin probiotic L. plantarum (PL-02) and designed different doses of probiotics to explore the benefits of improved exercise performance and anti-fatigue
Summary
In order to understand the effect of PL-02 supplementation on fatigue-related indicators and physiological adaptation after exercise, we collected blood samples after swimming for 10 min and resting for 20 min to analyze blood lactic acid, blood ammonia, and glucose. The sample was centrifuged at 1500×g at 4 °C for 10 min, and the serum was collected and measured by an automatic analyzer (Hitachi, Tokyo, Japan, Hitachi 7060). Parts of the muscle and liver tissues were stored in liquid nitrogen for glycogen content analysis, as previously described[29]. According to the method previously used in our laboratory, immediately after the euthanasia of the mice, the collected samples were stored at -80 °C for DNA extraction. Beta diversity was measured using PCoA-Unweighted UniFrac, which determines the difference of microbial composition between groups. Comparison of different groups was performed by t test with two-tailed and PERMANOVA analysis. A p value less than 0.05 were considered statistically significant
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