Abstract
Haemagglutination (HA) test for the detection of Newcastle disease virus (NDV) is described experimentally using chicken red blood cells (RBCs). The method proved to be reliable and effective, since infectious stocks of AF2240 strains of NDV were detected after the experimental procedure. To detect NDV in chicken allantoic fluids of SPF eggs, 0.1 mL of NDV AF2240 (velogenic) virus stock were inoculated into each egg. The virus was allowed to propagate for production of more stock and the unclarified allantoic fluids were harvested aseptically and tested for confirmation of the virus presence before the stock virus was further clarified and purified using high speed refrigerated centrifuge following sucrose gradients method. This sucrose gradients were later balanced with NTE buffer and sieved using 0.45 m filter and the purified samples of the virus were collected followed by HA test to detect the presence and to determine the virus titer. The results showed (28 = 256 HAU/mL) virus titer after purification of the allantoic fluids.
Highlights
Newcastle disease virus, known as avian paramyxovirus type-1, APMV-11,2 causes disease of Newcastle in aneclectic of birds, mostly noticed in chickens[3] and the disease is spread worldwide, affecting various species of poultry and other birds[4,5,6]
The agent that causes the disease is known as Newcastle disease virus (NDV), which is classified in the family of Paramyxoviridae[7,2] of the genus Avulaviru[8,9,10] and subfamily Paramyxovirinae[11]
The haemagglutination (HA) test used in our study with NDV AFF2240 strain showed effective, as the virus titer were obtained serologically using chicken red blood cells (RBCs) Fig. 1A
Summary
Known as avian paramyxovirus type-1, APMV-11,2 causes disease of Newcastle in aneclectic of birds, mostly noticed in chickens[3] and the disease is spread worldwide, affecting various species of poultry and other birds[4,5,6]. The agent that causes the disease is known as Newcastle disease virus (NDV), which is classified in the family of Paramyxoviridae[7,2] of the genus Avulaviru[8,9,10] and subfamily Paramyxovirinae[11]. NDV is classified into three types, based on its virulence strength (i.e. pathogenicity): Velogenic, causing gastrointestinal and neurological diseases resulting in high mortality rate of infected birds, Lentogenic, causing mild respiratory disease and Mesogenic, produces respiratory and nervous signs with moderate mortality[1]. The presence of numerous basic amino acids at the cleavage site of fusion protein (F0) precursor designates the molecular basis of virulence in chicken[5,18]
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