Effectiveness equilibration time on the quality of sperm buffalo in diluent tris yolk eggs with 4 % extract skin mangosteen (Garcinia mangostana L.)

Abstract

A comfortable livestock environment will produce good sperm productivity. Sperm dilution is done so that rural communities can feel the benefits. This study aims to determine the best equilibration time to produce high motility, intact plasma membrane values with low abnormality values in buffalo spermatozoa. This research used a completely randomized design (CRD) with four treatments and ten replications. Sperm came from three buffalo kept at the Ciawi Balitnak. The research treatment consisted of equilibration times of two, three, four and five hours. The parameters measured were sperm motility, abnormality, and intact plasma membrane (IPM). Equilibrium time does not affect buffalo sperm motility, abnormalities, and IPM. The range of motility, abnormalities, and IPM was 43.75%-55.00%; 20.25%-29.00%; 71.75%-74.75%. Conclusion; An equilibration time of two hours can maintain motility, abnormality, the intact plasma membrane of buffalo sperm with egg yolk tris diluent, and the addition of 4% mangosteen peel extract which was suitable for artificial insemination. The application can improve the standard of living of the community through increased meat production.